Influence of Hawthorn (Crataegus oxyacantha) Leaves Extract Administration on Myocardial Infarction Induced by Isoproterenol in Rats
Mona A. Sadek
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
Sahar Mousa
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
Shimaa El-Masry *
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
Amira M. Demain
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
*Author to whom correspondence should be addressed.
Abstract
Aims: To investigate the influence of ethanolic hawthorn leaves extract (EHLE) oral administration (200 mg / kg body weight /day) on isoproterenol (ISO) induced- myocardial infarction (MI) in rats and its bioactive constituents.
Methods: Healthy adult male albino rats (Sprague-Dawely strain) were divided into five groups (10 rats /group); MI was induced in rats by ISO at a dose of {85 mg/kg body weight/day subcutaneously (S.C.,)} on two consecutive days with a 24 hours interval.
Results: Pre-and post-treatment with EHLE significantly (p≤0.05) lowered the elevated serum cardiac enzyme marker activities namely, creatine kinase (CK), creatine kinase MB (CK- MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and specific MI markers level of galectin-3(Gal-3), cardiac troponin I (cTnI). Also, significantly (p≤0.05) ameliorated oxidant/ antioxidant status by decreasing oxidative stress biomarkers [tissue nitric oxide (NO), serum and tissue malondialdehyde (MDA)], and increasing antioxidant status [glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH)] in ISO-injected rats. Microscopic examination of heart tissues confirmed these results.
Conclusion: Our results demonstrated that EHLE has a cardioprotective effect against ISO-induced MI in rats due to its high antioxidant properties, inhibition of lipid peroxidation and restoration of cardiac enzyme activities.
Keywords: Isoproterenol, myocardial infarction, biomarkers, hawthorn, oxidative stress, antioxidant